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ywtE [2019-07-12 09:19:54]
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ywtE [2019-07-12 09:19:54]

dephosphorylation of the riboflavin precursor, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate (minor activity)
locus
BSU35850
pI
4.76
mw
31.44 kDa
protein length
286 aa Sequence Blast
gene length
861 bp Sequence Blast
function
dephosphorylation of the riboflavin precursor, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate (minor activity)
product
unknown
essential
no
ec
3.1.3.-
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.2|Biosynthesis/ acquisition of riboflavin/ FAD]

    • Gene

    Coordinates
    3,695,363 3,696,223

    The protein

    Catalyzed reaction/ biological activity

  • dephosphorylation of the riboflavin precursor, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate [Pubmed|26316208]

    • Protein family

  • [SW|HAD superfamily] [Pubmed|26316208]
  • [SW|Cof family] (according to UniProt)

    • Structure

  • [PDB|1NRW] ([protein|AF9523D3AA3C032B70175BCFFED34E9A42ED01AF|PhoC], 39% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|9730F7C18AC7CA68D144717851A0C261D9647E31|ywtE]
    description
    [Pubmed|22383849]
    view in new tab

    genes
    [gene|68FE85BAA457199D34C9068F38756972CD2D280D|tagT]-[gene|9730F7C18AC7CA68D144717851A0C261D9647E31|ywtE]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]

    • regulation

  • expressed at high salt concentrations ([protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]) [pubmed|18179421]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A546 (ywtE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/546 NBRP B. subtilis, Japan]
  • BKE35850 ([gene|9730F7C18AC7CA68D144717851A0C261D9647E31|ywtE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGTAGGGGAACCTCCT, downstream forward: _UP4_TAAAGAAAAGACTCCAGAGA
  • BKK35850 ([gene|9730F7C18AC7CA68D144717851A0C261D9647E31|ywtE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGTAGGGGAACCTCCT, downstream forward: _UP4_TAAAGAAAAGACTCCAGAGA

    • References

  • 26316208